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reagents Reagents

Limulus Amebocyte Lysate (LAL) Test

Turbidimetric Assay

The optical density (turbidity) increase that accompanies the clotting reaction is read in the Pyros Kinetix Flex tube reader or in a incubating microplate reader. The maximum sensitivity of 0.001 EU/mL can be achieved when uising ACC's Pyrotell-T reagent in our Pyros Kinetix or Pyros Kinetix Flex tube reader.

  • Requires the Pyros Kinetix Flex tube reader system or an incubating microplate reader
  • Maximum sensitivity to 0.001 EU/mL, highest level of sensitivity available in the LAL industry
  • Incubation time varies depending on the standard curve range
  • High sensitivity allows for greater dilution to overcome interference
  • High sensitivity allows for greater dilution to overcome interference
Pyrotell-T Pyrotell-T
Product Insert | Product Info Sheet

Product Description

Pyrotell-T lysate is used to quantify endotoxin in kinetic turbidimetric tests. Pyrotell-T lysate can be used with the Pyros Kinetix Flex tube reader and also incubating microplate readers. When used with the Pyros Kinetix Flex tube reader, Pyrotell-T gives a highly economic, flexible and sensitive LAL assay.

It can be used for a wide variety of endotoxin tests, ranging from standard water testing to samples requiring high sensitivity, such as intrathecal products and those requiring high dilutions to overcome interference.

Product Sensitivity

When used in a Pyros Kinetix tube reader, the maximum sensitivity is 0.001 EU/mL, which is 5 times more sensitive than any other LAL assays. The unique formulation of Pyrotell-T lysate allows a wide range of standard curves to be used, giving the user flexibility, speed, and ease in performing assays. When used in a microplate reader, the maximum sensitivity is 0.005 EU/mL.

Sample to Lysate Ratio

The ratio of sample: LAL is determined by personal preference and sample chemistry (interference patterns). A sample: LAL ratio for Pyrotell-T of 1:1 or 4:1 can be used according to user choice (and validation requirements).

Test Performance

The sample and lysate are added to test tubes or a microplate and incubated at 37C. The time of incubation is dependent on the lowest standard concentration in the standard curve. Software is used to construct the standard curve and calculate the endotoxin concentrations.

Product Stability

Once reconstituted, Pyrotell-T lysate is stable for 24 hours, if stored at 2-8C. Pyrotell-T lysate may be frozen once and will retain activity for as long as 3 months if stored at or below -20C.

Product Reconstitution

Pyrotell-T multi-test vials may be reconstituted with LAL Reagent Water (LRW), Pyrosol buffer or Glucashield buffer. Glucashield (1->3)--D-glucan inhibiting buffer is used to render the assay endotoxin specific.

Product Packaging

Pyrotell-T lysate is available in individual multi-test vials. Each vial contains reagent for approximately 96 tests.

5 mL/vial

#T0051-5 5 mL/vial 5 Pack (250 tests)
#T0051-25 5 mL/vial 25 Pack (1250 tests)

Designer: G. Pap
Hosted by: BcIS