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Glucatel Kit (1->3)-β-D-glucan Detection Product Insert | Product Info Sheet

The Glucatell assay kit is specific for (1->3)-β-D-glucan. The assay is based upon a modification of the Limulus Amebocyte Lysate (LAL) pathway. The Glucatell reagent is processed to eliminate Factor C, and is therefore specific for (1->3)-β-D-glucan. The reagent does not react to other polysaccharides, including beta-glucans with different glycosidic linkages.

Kinetic assay: 25 μL of sample or standard is mixed with 100 μL of reagent in a microplate.

End-point assay: 50 μL of sample is mixed with 50 μL of reagent in a microplate.

Kinetic assay: The Glucatell/sample mixture is incubated at 37°C ± 1°C in a microplate reader equipped with a 405 nm filter and analyzed over time by a suitable software program.

End-point assay: The Glucatell/sample mixture is placed in a microplate heating block at 37°C ± 1°C for the recommended time period. 50 μL each of the three diazo reagents are added to the mixture to stop the reaction, which is then read at 540-550 nm. Concentration of the samples is analyzed by a suitable software program.

Store all reagents at 2-8°C in the dark. Reconstituted Glucatell reagent should be stored at 2-8°C and used within 2 hours. Alternatively, reconstituted Glucatell reagent can be frozen at -20°C for 20 days, thawed once and used. The diazo-reagents should be used the day they are prepared.

The Glucatell kit is available as either an end-point or kinetic chromogenic assay for use in microplates. The kit contains the Glucatell reagent, a (1->3)-β-D-glucan standard, buffer, diazo reagents (end-point kit only), glucan-free water, and glucan-free microplates.

• Analyzing final products for (1->3)-β-D-glucan
• Investigating LAL Out of Specification results
• Qualifying raw materials
• Monitoring cellulosic filter extractables
• Monitoring fungal fermentation processes
• Analyzing fermentation and cell culture media
• Monitoring airborne glucan burden