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reagents Reagents

Limulus Amebocyte Lysate (LAL) Test

Gel Clot Assay

This is the simplest LAL test. The formation of a gel-clot indicates the presence of endotoxin in a sample. The method is performed in small test tubes and is read manually by inverting the test tubes. The maximum sensitivity is 0.03 EU/mL.

  • Requires non-circulating water bath or dry bath incubator
  • Manually read test
  • Reagents of differing sensitivity available: 0.25, 0.125, 0.06 and 0.03 EU/mL
  • May be less sensitive to interference than other LAL methods
Pyrotell Pyrotell
Product Insert | Product Info Sheet

Product Description

Pyrotell lysate was the first LAL gel-clot reagent licensed by the US FDA. It is easy to use and is available in both economical multi-test vials and convenient Single Test Vials (STVs). Pyrotell lysate is a robust reagent, producing firm, easily read clots and is resistant to interfering substances. The gel-clot test does not require sophisticated capital equipment and software and is the simplest LAL test to implement.

Product Sensitivity

Pyrotell lysate is available in multiple sensitivities: 0.03 EU/mL; 0.06 EU/mL; 0.125 EU/mL; 0.25 EU/mL. 0.5 EU/mL available in Single Test Vials only.

Sample to Lysate Ratio

For standard use, a 1:1 mixture of Pyrotell lysate to sample is used.

Test Performance

For 2 mL and 5 mL multi-test vials, 100 L of lysate is mixed with 100 L of sample in a reaction tube. For single test vials 200 L of sample is added to the vial, which serves as a reaction tube. Test tubes are incubated at 37C for 60 minutes 2 minutes. A positive test is indicated if the clot remains solid after the inversion of the test tube.

Product Stability

The reconstituted lysate is stable for 24 hours at 2-8C or for up to 3 months at or below -20C. May be frozen once and will retain activity for as long as 3 months if stored at or below -20C.

Product Reconstitution

Pyrotell multi-test vials may be reconstituted with LAL Reagent Water (LRW), Pyrosol buffer or Glucashield buffer. Glucashield (1->3)--D-glucan inhibiting buffer is used to render the assay endotoxin specific.

Product Packaging

Pyrotell lysate is available in single test vial (STV), 2 mL or 5 mL fill sizes. The 2 mL and 5 mL vials are sold individually. STVs are sold in packs of ten vials. Control Standard Endotoxin (CSE) is provided separately with a Certificate of Analysis, specific to the Pyrotell lysate lot with which it will be used.


Multi-Test
2 mL/vial

#G2003-5 0.03 EU/ml 5 Pack
#G2003-25 0.03 EU/ml 25 Pack
#G2006-5 0.06 EU/ml 5 Pack
#G2006-25 0.06 EU/ml 25 Pack
#G2125-5 0.125 EU/ml 5 Pack
#G2125-25 0.125 EU/ml 25 Pack
#G2250-5 0.25 EU/ml 5 Pack
#G2250-25 0.25 EU/ml 25 Pack

Multi-Test
5 mL/vial

#G5003-5 0.03 EU/ml 5 Pack
#G5003-25 0.03 EU/ml 25 Pack
#G5006-5 0.06 EU/ml 5 Pack
#G5006-25 0.06 EU/ml 25 Pack
#G5125-5 0.125 EU/ml 5 Pack
#G5125-25 0.125 EU/ml 25 Pack
#G5250-5 0.25 EU/ml 5 Pack
#G5250-25 0.25 EU/ml 25 Pack

Single Test Vial (STV)
0.2 mL/vial, 10 vials/pack

#GS003 0.03 EU/ml
#GS006 0.06 EU/ml
#GS125 0.125 EU/ml
#GS250 0.25 EU/ml


Pyrosate Pyrosate
Rapid Endotoxin Detection
Product Insert | Product Info Sheet

Product Description

The Pyrosate kit is developed as an easy-to-use LAL gel-clot test. The assay does not require special training or laboratory supplies. The step-by-step illustrated instructions allow the user to perform assays within minutes. The Pyrosate kit provides rapid results and is especially convenient for research, testing water and dialysate.

Product Sensitivity

The Pyrosate kit is available in sensitivities of 0.03 EU/mL, 0.125 EU/mL and 0.25 EU/mL. The test may be as short as 30 minutes, depending on the sensitivity.

Test Performance

The Pyrosate kit is the only rapid gel-clot test that contains a 2* endotoxin tube (PPC) matched to the sample (SPL) tube for each sensitivity. This feature is unique to the Pyrosate assay and ensures the validity of the test. The endotoxin tube (PPC) assures that the sample does not interfere with the test, ruling out false negatives. Pyrosate is formulated to eliminate false positives due to (1->3)--D-glucans. This endotoxin specific reagent does not require additional blocking buffers.

Product Stability

esting has demonstrated that the Pyrosate LAL reagent is stable at room temperature. This is an LAL product that does not require refrigeration for shipping or storage.

Product Packaging

The Pyrosate kit is available in two formats for each sensitivity, a 10 test kit and a 30 test bulk package. Each kit contains sample test tubes (SPL) and endotoxin test tubes (Positive Product Control-PPC).

Product Applications
  • Hemodialysis
  • Water and Water Systems
  • Filter Industry
  • Research
  • Final Product
Product Benefits
  • Shorter Assay Time
  • Endotoxin Specific
  • No Dilutions Required
  • No Refrigeration Required
  • Matched Positive Product Control
* (lambda) is the least concentration of endotoxin to cause a positive test result under standard conditions.
Pyrosate Kit
#PSD030-10 Pyrosate 0.03 EU/mL 10-Test Kit includes sample test tubes, positive product control test tubes, and disposable pipettes
#PSD125-10 Pyrosate 0.125 EU/mL 10-Test Kit includes sample test tubes, positive product control test tubes, and disposable pipettes
#PSD250-10 Pyrosate 0.25 EU/mL 10-Test Kit includes sample test tubes, positive product control test tubes, and disposable pipettes
#PSD030-30 Pyrosate 0.03 EU/mL 30-Test Bulk Package includes sample test tubes and positive product control test tubes only
#PSD125-30 Pyrosate 0.125 EU/mL 30-Test Bulk Package includes sample test tubes and positive product control test tubes only
#PSD250-30 Pyrosate 0.25 EU/mL 30-Test Bulk Package includes sample test tubes and positive product control test tubes only
#PPT50 Disposable transfer pipettes for Pyrosate kits, 50/pack


Designer: G. Pap
Hosted by: BcIS
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