The LAL reagent is formulated with a synthetic substrate which produces a chromophore when cleaved by endotoxin activated enzyme. The test is read at 405 nm, usually in a microplate reader. The maximum sensitivity of 0.001 EU/mL can be achieved when using ACC's Pyrochrome Chromogenic reagent with Glucashield buffer.
- Requires a tube reader or incubating microplate reader (an incubating reader is required for the kinetic method)
- Maximum sensitivity to 0.001 EU/mL when using ACC's Pyrochrome reagent with Glucashield Buffer
- A quantitative assay providing electronic stored data and print-outs of results
- Incubation time varies depending on the standard curve range
- Higher sensitivity allows for greater dilution to overcome interference
- The option of a diazo-coupled endpoint method (read at 540-550 nm) is available, which is useful for samples that absorb light at 405 nm
Chromo-LAL lysate is lyophilized with substrate reagent and buffers. It is optimized for the kinetic chromogenic LAL test method in microplate readers. Chromo-LAL is a buffered, stable and robust lysate, suitable for quantitative testing of a wide range of samples.Product Sensitivity
The sensitivity for chromogenic assays is determined by the lowest standard concentration on the standard curve used for the assay. The maximum sensitivity of Chromo-LAL is 0.005 EU/mL.Sample to Lysate Ratio
In the Chromo-LAL test, reconstituted Chromo-LAL reagent is used at a ratio of 1:1 and a volume of 100 ìL.Test Performance
The Chromo-LAL/sample mixture is incubated at 37 ± 1°C in a microplate reader equipped with a 405 nm filter. Software is used to construct the standard curve and calculate the endotoxin concentrations.Product Stability
The reconstituted lysate is stable for 24 hours at 2-8°C or for two weeks at -20°C or colder if frozen immediately after reconstitution. Reconstituted Chromo-LAL lysate may be frozen and thawed once.Product Reconstitution
Chromo-LAL lysate is reconstituted with LAL Reagent Water (LRW). It can also be reconstituted with Glucashield (1->3)-â-D-glucan inhibiting buffer, to render the assay endotoxin specific.Product Packaging
Chromo-LAL lysate is sold by the vial for greater ordering and testing flexibility. This allows you to order the amount of lysate that is right for you.
|#C0031-5 ||3.2 mL/Vial 5 Pack (150 tests) |
|#C0031-25 ||3.2 mL/Vial 25 Pack (750 tests) |
Pyrochrome is a versatile quantitative reagent that is used to perform kinetic or endpoint assays. It is a sensitive and flexible reagent that can be used for testing in compliance with the USP, EP and JP bacterial endotoxins test chapter. Pyrochrome is offered with either Pyrochrome Buffer or for endotoxin-specific testing, with Glucashield Buffer. Pyrochrome can be used with the new Pyros Kinetix Flex tube reader at a 1:1 and economical 4:1 ratio.
Pyrochrome can be used for a wide variety of endotoxin tests, ranging from standard water testing to samples requiring high sensitivity, such as intrathecal products and those requiring high dilutions to overcome interference. The standard Pyrochrome test is read at 405-410 nm.
It is also offered in a diazo kit for endpoint tests. (The diazo reagents shift the absorption spectrum making it especially useful for testing samples with color interference.) The test is read at 540-550 nm.Product Sensitivity
The sensitivity for chromogenic assays is determined by the lowest standard concentration on the standard curve used for the assay. The maximum sensitivity of Pyrochrome is 0.001 EU/mL when run in Pyros Kinetix Flex tube reader or incubating microplate reader with Glucashield Buffer.Sample to Lysate Ratio
Reconstituted Pyrochome can be used at an economical ratio of 4:1 and a volume of 50 ìL/well (60 test) in the Pyros Kinetix Flex tube reader. Pyrochrome LAL reagent can also be used at a ratio of 1:1 and a volume of 100 ìL/well (30 tests/vial) or 50 ìL/well (60 tests/vial) in a microplate reader.Test Performance
The Pyrochrome/sample mixture is incubated at 37±1°C and read in a microplate reader equipped with a 405-410 nm filter or 540-550 nm filter for diazo method. The time of incubation is dependent on the lowest standard concentration in the standard curve. Software is used to construct the standard curve and calculate the endotoxin concentrations.Product Stability
Once reconstituted, Pyrochrome is stable for 8 hours when stored at 2-8°C.Product Reconstitution
Pyrochrome lysate is reconstituted with an optimized Pyrochome reconstitution buffer (C1500). Pyrochrome can also be reconstituted with Glucashield buffer, a (1->3)-â-D-Glucan inhibiting buffer, to render the assay endotoxin specific (CG1500).Product Packaging
Pyrochrome is offered with a choice of reconstitution buffer and is recommended for use with the 10 ng/vial Control Standard Endotoxin (CSE, EC010).
|#C1500-5 ||3.2 mL/Vial 5 Pack (300 tests) |
|#C1500-25 ||3.2 mL/Vial 25 Pack (1500 tests) |
|#CD060 ||60 Test Kit (diazo format) |
|#CG1500-5 ||3.2 mL/Vial 5 Pack (300 Test with Glucashield Buffer) |
|#CG1500-25 ||3.2 mL/Vial 25 Pack (1500 Test with Glucashield Buffer) |